| 1826–1828 | Rev. Jasper Adams |
| 1829 | [none] |
| 1830–1835 | Rev. Richard S. Mason |
| 1836–1858 | Rev. Benjamin Hale |
| 1858–1862 | Rev. Abner Jackson |
| 1863–1864 | Rev. Henry A. Neeley |
| 1865–1867 | Rev. O. Pelham Williams |
| 1867–1869 | [none] |
| 1869–1871 | Rev. James Rankine |
| 1871–1874 | Rev. Maunsell Van Rensselaer* |
| 1875–1882 | Rev. Walter Ayrault |
| 1883–1887 | Rev. William Mortimer Hughes |
| 1887–1897 | Rev. Rob Roy MacGregor Converse |
| 1897–1910 | Rev. Joseph Alex |
| 1911–1913 | Rev. George Grey Ballard, Jr. |
| 1913–1928 | Rev. John Brewster Hubbs |
| 1928–1939 | Rev. Irving Angell McGrew |
| 1939–1945 | Rev. Stuart Gottlieb Cole |
| 1945–1950 | Rev. David Random Covell |
| 1951–1956 | Rev. Allen Floyd Kremer |
| 1957–1962 | Rev. David Archelaus Crump |
| 1962–1966 | Rev. R. Channing Johnson |
| 1966–1967 | Rev. Charles W. Scott |
| 1967–1977 | Rev. Durstan R. McDonald |
| 1977–1978 | Rev. James H. Littrell |
| 1979–1984 | Rev. Ellen K. Wondra |
| 1984–1994 | Rev. Frederick P. Moser |
| 1994–2015 | Rev. Lesley Adams |
| 2015–2018 | Rev. Maurice Charles |
| 2019–Present | Rev. Nita Byrd |
*Indicates Acting or Interim Chaplain